Monday, May 5, 2014
Journal May 5, 2014,- cinco de mayo - Tosca in vitro
The above photo shows one of the Tosca explants today, less than two weeks in culture. The small V shaped growth on the left side of the plant is a node that is now forming two distinct leaves.
All of the explants taken on that date are showing good progress, including the Arbosana, which I had just about given up on.
For this protocol I decided to go very light on salts and hormones, using one half MS salts, one hormone and slightly more sugar.
Reading several abstracts on olive micro-propagation I found some were propagated using salts intended for walnuts and some for almonds. From what I have read, a protocol that works with one variety may not work with another. That said, I guess I was fortunate that both varieties of olive seem to be responding to my test protocol.