Wednesday, May 16, 2018

Journal May 16. 2018 - Phalaenopsis seed starting progress

Today I noticed a tiny spot of what I thought was mold in one of the flasks.  Already I had discarded one flask because of mold, and both flasks contained the seeds from the dry pod.  That appears to confirm that the chance of contamination is greater with the dry pod seeds.

The seeds from green pod are really making progress now, beginning to form protocorms, which will form tiny orchid plants, thousands of tiny orchid plants.

Not wanting to give up on the flask with the mold I decided to attempt to rescue the forming protocorms.   An experimental batch of media was prepared using 1/4 Murashige and Skoog with vitamins, distilled water, coconut water, agar, and sugar.  

Under sterile conditions, small clumps containing the protocorms were transferred to the prepared media.  Now, it is a matter of waiting to see if mold begins to form in these vessels. 
 


 

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