Thursday, June 12, 2014
Journal June 12, 2014 - Tosca olive explant
Above is photo of a Tosca olive cutting that has now been in vitro for about seven weeks. At this point I regret that I did learn micro-propagation techniques much sooner and did not waste time, or resources, on traditional cloning. As is clear in the photo, the explant is developing a very nice root system.
Two ounces of protocol were added to each jar to give the plantlets plenty of room to develop a root system during this phase of the process.
The next phase of the process will be to transfer the plantlets to a sterile mixture of coir and perlite and begin the acclimatization process.
As of today I have several varieties responding to tissue culture, some better than others, however, I am certain at this point that all will be successful.