Journal June 13, 2014 - Picholine in vitro

A small section of branch was removed from the Picholine olive for cloning.  After it was cleaned and rinsed the section was cut into three single node pieces that were then placed into culture media.

Rather than placing each explant in an individual jar, I have elected to use a single jar to save space and media.  When, and if, the plants begin to respond they will be placed in individual jars for rooting.

The Tirilye seedling is almost entirely acclimated to a normal environment, at this point it is spending about 75% of its time in the greenhouse.